Gene transfer in Neisseria gonorrhoeae.

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Gene transfer in Neisseria gonorrhoeae.

The ability of bacteria to exchange deoxyribonucleic acid (DNA) endows these organisms with greater genetic variability and increased capability to adapt to changing environments. Many bacteria have evolved transformation and conjugation systems to effect this exchange, whereas others achieve it through the action of bacteriophages. In Neisseria gonorrhoeae no transducing bacteriophages have be...

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Plasmid-mediated chromosomal gene transfer in Neisseria gonorrhoeae.

An indigenous Neisseria gonorrhoeae conjugative plasmid, pLE2450, was tested for its ability to mediate chromosomal gene transfer between gonococcal strains. Plasmid-mediated chromosomal transfer was detected at a low frequency and can be used to establish certain linkage relationships between amino acid and antibiotic resistance markers.

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Transfer of beta-lactamase plasmids by conjugation in Neisseria gonorrhoeae.

Conjugation experiments in vitro have been used in an attempt to explain certain epidemiological phenomena seen amongst penicillinase producing Neisseria gonorrhoeae (PPNG) isolated at St Mary's Hospital, London. These include changes in plasmid profiles, a lack of beta-lactamase encoding plasmids in PAOU-requiring strains and a difference in the serological classification of strains of PPNG an...

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Neisseria gonorrhoeae

Two fi-lactamase-producing strains of Neisseria gonorrhoeae were studied. The substrate profile, molecular weight, and isoelectric point of their ,8-lactamases were similar to those of the TEM-1 enzyme produced by many gram-negative bacilli. The gonococcal f8-lactamase was cell bound during exponential growth and was most likely located in the periplasm. Penicillin hydrolysis was efficient in i...

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Expression of Type IV pili by the bacteria! pathogen Neisseria gonorrhoeae appears to be essential for colonization of the human host. Several N. gonorrhoeae gene products have been recently identified which bear homology to proteins invoived in pilus assembly and protein export in other bacterial systems. We report here the isolation and characterization of transposon insertion mutants in N. g...

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ژورنال

عنوان ژورنال: Clinical Microbiology Reviews

سال: 1989

ISSN: 0893-8512,1098-6618

DOI: 10.1128/cmr.2.suppl.s24-s28.1989